Rp-hplc Method for the Simultaneous Estimation of Atrovastatin and Losartan in Pure and Tablet Formulations

The combination of Atorvastatin and Losartan was used in the management of Hypertension. The present work was focused on the development of RP-HPLC method for the estimation of Atorvastatin and Losartan in binary mixtures by internal standard method. The separation was performed on LUNA C18 column at 238 nm by isocratic elution using Telmisartan as internal standard. Methanol and phosphate buffer (pH 6.8) in the ratio of 80:20 with a flow rate of 1ml/min at ambient temperature was found to be suitable for chromatographic separation. Atorvastatin and Losartan exhibits linearity in the range of 60-120 and 96-180 μg/ml respectively. The proposed method evidenced the absence of chromatographic interference by pharmaceutical excipients. Separation of the mixtures was proved to be good from the resolution of the peaks. Validation and recovery studies were performed and the results proved the method is suitable for routine analysis.